Methods
In-gel protein digestion (protocol from Bruker)
SYPRO Ruby gel staining (protocol from Invitrogen)
iTRAQ labeling of peptides (protocol from Applied Biosystems)
Enhanced Coomassie staining protocol: PMID: 19684561
In-gel protein digestion (protocol from Bruker)
SYPRO Ruby gel staining (protocol from Invitrogen)
iTRAQ labeling of peptides (protocol from Applied Biosystems)
Enhanced Coomassie staining protocol: PMID: 19684561